单细胞专栏-如何给orig.ident换名字

##官网上回答问题的作者给予的回答
> # Quickly preview the metadata
> head(x = pbmc_small[[]])
                  orig.ident nCount_RNA nFeature_RNA RNA_snn_res.0.8
ATGCCAGAACGACT SeuratProject         70           47               0
CATGGCCTGTGCAT SeuratProject         85           52               0
GAACCTGATGAACC SeuratProject         87           50               1
TGACTGGATTCTCA SeuratProject        127           56               0
AGTCAGACTGCACA SeuratProject        173           53               0
TCTGATACACGTGT SeuratProject         70           48               0
               letter.idents groups RNA_snn_res.1
ATGCCAGAACGACT             A     g2             0
CATGGCCTGTGCAT             A     g1             0
GAACCTGATGAACC             B     g2             0
TGACTGGATTCTCA             A     g2             0
AGTCAGACTGCACA             A     g2             0
TCTGATACACGTGT             A     g1             0
> # Create a new metadata column called "original" with the contents of "orig.ident"
> pbmc_small$original <- pbmc_small$orig.ident
> # Remove the "orig.ident" metadata column
> pbmc_small$orig.ident <- NULL
> # Quickly preview the metadata again
> head(x = pbmc_small[[]])
               nCount_RNA nFeature_RNA RNA_snn_res.0.8 letter.idents groups
ATGCCAGAACGACT         70           47               0             A     g2
CATGGCCTGTGCAT         85           52               0             A     g1
GAACCTGATGAACC         87           50               1             B     g2
TGACTGGATTCTCA        127           56               0             A     g2
AGTCAGACTGCACA        173           53               0             A     g2
TCTGATACACGTGT         70           48               0             A     g1
               RNA_snn_res.1      original
ATGCCAGAACGACT             0 SeuratProject
CATGGCCTGTGCAT             0 SeuratProject
GAACCTGATGAACC             0 SeuratProject
TGACTGGATTCTCA             0 SeuratProject
AGTCAGACTGCACA             0 SeuratProject
TCTGATACACGTGT             0 SeuratProject
##You can add metadata columns using AddMetaData, the [[ operator, or the $ operator. For your specific case, I would create a character vector of 'ctrl' or 'patient' based on testing the value of your metadata column, then add it in like I did above.

##load library,先加载一堆有的没的包，防止后面代码找不到函数，没办法调取
library(Seurat)
library(dplyr)
library(reticulate)
library(sctransform)
library(cowplot)
library(ggplot2)
library(viridis)
library(tidyr)
library(magrittr)
library(reshape2)
library(readxl)
library(progeny)
library(readr)
library(stringr)
##先读取xlsx格式的表格
metatable <- read_excel("id_change.xlsx")
metadata <- FetchData(seu_obj, "orig.ident")
metadata$cell_id <- rownames(metadata)
metadata$sample_id <- metadata$orig.ident
##left_join：左连接，保留X中得所有观测，这样可以保留原表数据
metadata <- left_join(x = metadata, y = metatable, by = "orig.ident")
rownames(metadata) <- metadata$cell_id
##AddMetaData：添加元数据列；AddMetaData()中传递给元数据参数的内容必须是具有与数据@meta.data中的行名匹配的行名的数据框
seu_obj <- AddMetaData(seu_obj, metadata = metadata)
##查看矩阵情况
head(seu_obj)
##发现多了original、sample_id、cell_id、Groups几列，需要把original替换为orig.ident
##如果是cell_id及sample_id自己研究不在需要，可以跳过那两行代码
##删去orig.ident行，参考seurta官网答疑的代码
seu_obj$orig.ident <- NULL
##新生成orig.ident的一列
seu_obj$orig.ident <- seu_obj$original
##删去original列
seu_ob$original <- NULL
##查看seurat对象情况
seu_obj
##选择新给予的分组进行test，看看能不能有图出来，表明改的是合理的
DimPlot(seu_obj, group.by = "orig.ident",  pt.size = 0.1)